Clinical Comparative Study for Tannic Acid and Vitamin C Activity as Antioxidants

Antioxidants are the substrates which can prevent free radicals generation and protect the cells from membrane oxidation that cause cells damage and tissue injury. In this study, we attempted to compare between tannic acid (TA) and ascorbic acid (AS) antioxidative activity in vitro. The study included fifty healthy subjects, and serum lipid peroxidation (LPO) was promoted by incubation with copper ions(copper sulphate).The results illustrated that the optimum concentration of copper to promote lipid peroxidation in sera was (0.1 M) with incubation at 37°C for (1 hr). The concentrations of (AS) (10-5and 10-4 M) could not inhibit (LPO), whereas the concentrations (10-3, 10-2, 10-1, 0.2) had a certain effect on (LPO) and could not decrease malondialdehyde (MDA),LPO marker, levels to the normal values with negative correlation coefficient ( r= -0.72 ).Yet all TA concentrations(10-5, 10 -4, 10-3, 10-2, 10-1, 0.2, 0.3)M were able to inhibit (LPO) and decrease (MDA) levels to the normal values at concentrations (10-1 M and up ), also TA concentrations were correlated negatively with ( MDA) levels (r =- 0.90).