PCR Detection of Intimin Gene in Shiga-Like Toxin Producing Escherichia Coli Isolated from Animal Carcasses

The Shiga- like toxin-producing Escherichia coli (STEC) is important group of food-bornepathogens. These bacteria can cause severe health problems in humans like diarrhea,hemorrhagic colitis and hemolytic uremic syndrome.Cattle, sheep and goat are thought to be a reservoir for E. coli(STEC). Number of samples collected 117 and 94 animals carcasses swaps (cattle and sheep) respectively. Total number n of sorbitol fermented E coli 85 (40.284%) out of 211 samples from cattle and sheep. Sheep carcasses has the higher rate (50%).17 samples (20%) STEC out of 85 NSREC isolates were identified. Cattle carcass has the higher rate (28.94%).Total 17 of STEC samples tested by serological latex test, 14 (6.635% was serotypes O157 and O157:H7 STEC. All 14 isolates tested with PCR technique were -ve for the presence of intemin gene (eaeA) that responsible to attachment of bacteria to intestinal cells