• شماره ركورد
    94945
  • عنوان مقاله

    Extraction and Purification of Cellulase from Ruminants Rumen Liquor

  • پديد آورندگان

    AL-Rawi, Rasha Mohammed Shaker University of Baghdad - College of Dentistry, Iraq

  • از صفحه
    1
  • تا صفحه
    8
  • تعداد صفحه
    8
  • چكيده عربي
    Producing cellulase by rumen cellulytic microorganisms was extracted and purified by dialysis and DEAE-Sephadex A50 Ion-exchange chromatography 8.53 fold with a 40.02 % yield and a specific activity of 718.4 U/mg. It was optimally active at pH 6 and 40 °C while stable from pH 5 to 7 and 20 to 40 °C. A 3, 5 - Dinitro Salicylic Acid (DNSA) solution was used for measuring cellulase activity at 575 nm.
  • كليدواژه
    Extraction and Purification , Cellulase , Ruminants , Rumen Liquor
  • سال انتشار
    2011
  • عنوان نشريه
    مجله الانبار للعلوم الزراعيه
  • عنوان نشريه
    مجله الانبار للعلوم الزراعيه