Determination of Success Criteria in Transient Thermal Stresses: A Biomechanical Case Study of Cell Cryopreservation

Determination of Success Criteria in Transient Thermal Stresses: A Biomechanical Case Study of Cell Cryopreservation

This research proposes success criteria for performance evaluation of biological transient thermal stresses events. A literature review is conducted in area of biological phenomenological processes in order to classify the approaches for success criteria determination and their merits. Biological environment is complex in correlated occurrence of microscopic and macroscopic phenomena. Therefore the modeling of this complex medium, in context of mechanical stresses, requires numerical solution of conservation equation and inclusion of corresponding constitution models. Determination of success criteria is a challenging object, and requires consideration of several dependent figures of merits (e.g. temperature, mass and etc.). The developed success criteria matrix is based on the approaches of representation of the given figures of merit in the problem. A multi objective criteria is developed according to the phenomena occurrence in the intended study and the selected proper figures of merit. The matrix determines the region of acceptance as well as the rejection area. The methodology is applied to the case of cell cryopreservation phenomenon. The process of freezing in living cells is considerably more complicated due to the presence of cellular structure. Since the process is a transient thermal stress on the cell structure, the success criteria is determined based on two figures of merit of temperature and its rate of change. A limited numerical calculation is completed for study of the case thermal behavior. The uncertain parameters are considered random and Monte Carlo simulation is conducted for inclusion of their variation in the calculation. The situation is specified for the observation of the success criteria and occurrence of the failure