Paclitaxel Loaded Niosome Nanoparticle Formulation Prepared via thin film hydration technique: An in vitro Evaluation on human Gastric carcinoma

Niosomes are nano vehicles utilized in drug delivery systems. Niosomes are prepared by various methods. In this study, Paclitaxel loaded Niosomes were prepared by thin film hydration method. The characterization tests that included dynamic light scattering (DLS) and UV-Vis spectrophotometry were employed to evaluate the quality of the nanocarriers. Percent of Encapsulation Paclitaxel prepared with Tween 60 and cholesterol was 99.4% .In addition, the polydispersity index, mean size diameter and zeta potentials of Niosomal Paclitaxel nanoparticles were found to be 0.203, 119.7 nm and -4, respectively. Cytotoxicities of Niosomal Paclitaxel nanoparticles and free paclitaxel on AGS cell line after 48 hours were studied by MTT assay to determine cell viability. The results demonstrated that a 1.5-fold reduction in Paclitaxel concentration was measured when the Paclitaxel administered in NanoNiosome compared to Free Paclitaxel solution in AGS cells