Synthesis of Novel Fluorescein derivatives for Protein Labeling

Life is dependent on the chemistry of proteins. Proteins that are made up of 20 aminoacids, carry out fundamental reactions such as glucose and lipid break down. Therefore to be able to label proteins would give an enormous advantage in manipulating protein structure and function.In this work, we have aimed at using bioorthogonalchemistry to label proteins using fluorescein derivative in such a way that protein function and structure would not be altered dramatically [1]. In our preliminary experiment, we synthesis 5(6)- carboxyfluorescein with resorcinol and trimelliticanhydride [2]. We modified the 5(6)- carboxyfluorescein to 5(6)-acidchloridefluoresceinwith chlorinating agent. 1HNMR were used to confirm the products. The product were used to label proteins in physiological conditions. The label protein was analysed via gel electrophoresis. Our future work, will focus on protein aminoacids that have reacted with fluorescein derivative. In addition, different proteomes will be used for binding new involving proteins.