Synthesis of Silver Nanoparticles via Poly (Acrylamide), Chitosan g-poly (Acrylamide) Hydrogel Networks by use of NaBH4 and Glucose as Reducing Agent and Use them for Antibacterial Application

Nanoparticles (NPs) and nanostructured materials areconsidered as an important class of materials that have foundfascinated interest in biomedical, catalytical, opticaland electronic as well as quantum-size domain applications. A number of methods have been suggested anddeveloped for the synthesis of non-agglomerated nanoparticles. These include polymers, biological macromolecules, latex particles, mesoporous inorganicmaterials dendrimers, microgels or hydrogels, colloidal systems, and others. Most of theabove mentioned systems provide a good stability for nanoparticles, but microgel or hydrogel network template/carriersystems can act in a similar way. In addition, these make itmore convenient for producing smaller size nanoparticles.However, these make it more convenient for producing smallersize nanoparticles [1]. Poly (Acrylamide),Chitosan g-poly (Acrylamide) hydrogel had synthesized according to Kim et al [2] and Pourjavadi et al [3]. Briefly after synthesizing hydrogels they placed in AgNO3 solution for 24 hours and after that they transferred to NaBH4 and Glucose with NaOH solutions with different concentrations. Effect of hydrogel kind, reducing agent species and their concentrations on the size of silver nanoparticles have been studied. The formationof silver nanoparticles has been confirmed with ultraviolet visible (UV–Vis) spectroscopy, dynamic light scattering (DLS), TEM, SEM, Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) analyses. Thermogravimetric analysis (TGA)provides the amounts of silver nanoparticles exist in the hydrogel networks. In conclusion, we have demonstrated that controlled sized silver nanoparticles with 10-40 nm were synthesized inside the hydrogeltemplates. The nano-composites exhibitfair anti-bacterial action against E. coli and B.cereus as model bacteria.