Phytochemical study and evaluation the effects of different alcoholic extracts of turmeric on colon cancer cells HT29

Turmeric is commonly known for its medicinal values in the majority of traditional medicinesystems.Curcumin is a main coloring substance in Curcuma longa and two related compounds, demethoxycurcumin and bisdemethoxycurcumin, are altogether known as curcuminoid.Curcumin, the most active curcuminoid found in Turmeric, has been shown to possess a multitude of beneficial effects in the treatment of cancers, cardiovascular disease, and inflammation [1]. The goal of this studying is evaluation of anticancer and anti-oxidant properties of Turmeric (Curcuma longa) on colon cancer cells HT29 and phytochemical analysis.The phytochemicals of turmeric have been identified by phytochemical methods [2]. The phytochemical tests have been done in this study are alkaloid, glycoside, flavonoid, tannin, saponin and fixed oils.In order to evaluate cytotoxic and anti-oxidant characteristics, ethanolic extracts from Turmeric has been obtained, using maceration, Soxhlet extraction, ultra-sonic assisted extraction techniques. The effects of these extracts on HT29 have been evaluated using MTT and NBT tests. The results of photochemical showed that alkaloid, glycoside, flavonoid and fixed oils compounds, but no saponins and tannins are detected. The results of MTT showed the least cell survival value of treated cancerous cell was concerned to Soxhlet apparatus. Furthermore, the results of this research showed that the Turmeric extract by ultra-sonic displays peroxidant characteristics and drives the cancerous cell toward apoptosis. The existence of numerous compounds ethanolic extracts from Turmeric have been identified anti-oxidant and peroxidative properties of different extracts of Turmeric.