INHIBITORY EFFECTS OF THE ESSENTIAL OIL OF CHAMOMILE (Matricaria chamomilla L.) FLOWER ESSENTIAL OIL ON THE GROWTH OF Aspergillus flavus

INHIBITORY EFFECTS OF THE ESSENTIAL OIL OF CHAMOMILE (Matricaria chamomilla L.) FLOWER ESSENTIAL OIL ON THE GROWTH OF Aspergillus flavus

The antifungal activity of essential oil from Matricaria chamomilla L. on Aspergillus flavus growthhas been determined. In order to identify the chemical composition of essential oil of Matricaria chamomilla, theflowers of this plant which grows in a village in Shiraz Province at full flowering stage in April 2016 were collected. The sample was cleaned and then dried in the shade, and essential oil hydrodistillation method was performed. The main oil content from the plant of M. chamomillawas1.45%(v/w) and that essential oil was analyzed by capillary gas chromatography (GC) using flame ionization (FID) and capillary gas chromatography coupled mass spectrometry (GC/MS) for detection. Twenty-sixcompounds were identified in the essential oil ofM. chamomillathat included 98.57% of the total oil. The major components wereα-bisabolol (46.28%), Trans-trans-farnesol (14.52%), Cis-β-farnesene (8.37%) and Guaiazulene (4.76%). The study of the antifungal effects of the oil sample was carried against strain of Aspergillus flavus (PTCC=5004) by disc diffusion method via average inhibition zone.The results show that the essential oil from the plant of M. chamomillaat 1, 1/2 and 1/4 oil dilutions exhibited strong antifungal activity than Streptomycin sulphate (72% SP) and gentamycin (8 mg/ml) antibiotics on A. flavus.A large percentage antifungal activity of chamomile oil is related to α-Bisabolol a natural plant sesquiterpene alcohol is the main compound with fungicidal properties.

The antifungal activity of essential oil from Matricaria chamomilla L. on Aspergillus flavus growthhas been determined. In order to identify the chemical composition of essential oil of Matricaria chamomilla, theflowers of this plant which grows in a village in Shiraz Province at full flowering stage in April 2016 were collected. The sample was cleaned and then dried in the shade, and essential oil hydrodistillation method was performed. The main oil content from the plant of M. chamomillawas1.45%(v/w) and that essential oil was analyzed by capillary gas chromatography (GC) using flame ionization (FID) and capillary gas chromatography coupled mass spectrometry (GC/MS) for detection. Twenty-sixcompounds were identified in the essential oil ofM. chamomillathat included 98.57% of the total oil. The major components wereα-bisabolol (46.28%), Trans-trans-farnesol (14.52%), Cis-β-farnesene (8.37%) and Guaiazulene (4.76%). The study of the antifungal effects of the oil sample was carried against strain of Aspergillus flavus (PTCC=5004) by disc diffusion method via average inhibition zone.The results show that the essential oil from the plant of M. chamomillaat 1, 1/2 and 1/4 oil dilutions exhibited strong antifungal activity than Streptomycin sulphate (72% SP) and gentamycin (8 mg/ml) antibiotics on A. flavus.A large percentage antifungal activity of chamomile oil is related to α-Bisabolol a natural plant sesquiterpene alcohol is the main compound with fungicidal properties.