شماره ركورد كنفرانس :
3926
عنوان مقاله :
Performance characterization of a low-cost dual-channel camera-based microarray scanner
پديدآورندگان :
Akhoundi Farhad akhoundi@alum.sharif.edu DNA Microarray Analysis Lab Sharif University of Technology Tehran, Iran , Ghobeh Maryam ghobe@vaslab.ir DNA Microarray Analysis Lab Sharif University of Technology Tehran, Iran , Ghiasvand Ehsan ghiasvand@aut.ac.ir DNA Microarray Analysis Lab Sharif University of Technology Tehran, Iran , Akbari-Roshan Kamyar akbariroshan kamyar@ee.sharif.edu DNA Microarray Analysis Lab Sharif University of Technology Tehran, Iran , Motahari Seyed Abolfazl motahari@sharif.edu DNA Microarray Analysis Lab Sharif University of Technology Tehran, Iran
كليدواژه :
DNA microarray , camera , based scanner , CCD and CMOS sensor , fluorescence microscopy and imaging , Cy3 and Cy5 dyes.
عنوان كنفرانس :
بيست و چهارمين كنفرانس مهندسي برق ايران
چكيده فارسي :
In this paper, we have proposed, designed, implemented, and characterized a low-cost camera-based microarray scanner which is capable of imaging fluorescently-labeled DNA or Protein microarrays. The proposed system is designed to simultaneously measure two different fluorescent dyes using two parallel channels which increase the overall scan speed. We have shown that the wide dynamic range of system makes it able to detect fluorophore densities from 100 − 106 molecule/µm2. In each capture, a 5.6 mm × 3.7 mm field is imaged on a 22.3 mm × 14.9 mm (18 megapixels) CMOS sensor. Therefore, the microarray can be scanned with∼1µm2 spatial resolution which is high enough to distinguish borders of spots with less than 50µm diameter. To characterize the scanner performance, we have used molecules labeled with fluorescent cyanine dyes, including Cy3 andCy5;however,theproposedscannercanbemodifiedtodetect any other fluorescent dye with emission spectra within 400-800 nm. We believe it is the most cost-efficient microarray scanner ever achieved.
