پديدآورندگان :
Khataee Alireza a_khataee@yahoo.com Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran , Haddad Irani Nezhad Mahsa - Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran , Hassanzadeh Javad - Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran
چكيده فارسي :
Peroxidase enzymes is a wide family of enzymes which are the hydrogen peroxide reduction catalyzers and changing of it to hydroxyl radical (OH•) [1]. Here, we applied WS2 NS as a good nominate to study its peroxidase-like feature due to their considerable advantages comparing with natural enzyme. This advantages are high stability, low preparing cost, simple conservation, being flexible compound and designing structure, and finely catalytic activity [2]. WS2 NS were synthesized by using a combined method including ultrasonic and hydrothermal processes and characterized using X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), and transmission electron microscopy (TEM) analysis. It is observed that the synthesized WS2 NS have a great mimetic activity and could strongly promote the chemiluminescence (CL) emission intensity of NaHCO3-H2O2 reaction. The intensity of CL emission was linearly proportional to H2O2 concentration and, based on this effect, a novel and sensitive CL probe was developed for rapid H2O2 determination. The effect of important factors including the concentration of reagents and WS2 NS were optimized. Under the selected operating condition, a linear calibration graph was obtained in the range of 0.005-20 µM for H2O2 with a detection limit of 1.8 nM. The proposed method showed good features for H2O2 determination and was satisfactorily exploited for H2O2 evaluation in some real samples.
