چكيده فارسي :
The identification of new tools able to recognize ultratrace amounts of CA125 with high efficacy and sensitivity is an important challenge in oncology [1]. The development of a novel flexible and ultrasensitive aptasensor based on carboxylated multiwalled carbon nanotubes (MWCNTs)/ reduced graphene oxide-based field effect transistor (FET) has been reported for lable-free detection of the ovarian cancer antigen 125 (CA125). The fabricated sensor has a straightforward design based on the noncovalent attachment of MWCNTs/aptamer conjugated onto few layers reduced graphene oxide nanosheets and its integration with poly- methyl methacrylate (PMMA) as a suitable platform for designing flexible field-effect transistors. The surface properties of the aptasensor were characterized using scanning electron microscopy (SEM), Transmission electron microscopy (TEM) and atomic force microscopy (AFM). Under optimal conditions, the proposed aptasensor exhibited a wide linear dynamic range for CA125 (1.0×10-9-1.0 U/mL) with a low detection limit of 5.0×10-10 U/mL. The proposed aptasensor was also successfully applied to detect CA125 in real human serum samples. In addition, the aptasensor exhibited excellent mechanical bending durability and showed high sensitivity, selectivity, stability and reproducibility which offer great promise as a high performance and flexible FET-type aptasensor to detect CA125 and other cancer biomarkers in clinical samples and biological fluids.
