Atenolol drug enantiomers separated and measured by high performance liquid chromatography column and mobile phase chiral Ghyrkayral

In this research work, Enantiomers (R)- Atenolol and (S)-Atenolol were separated on the column μBondapak C18 (mm5, mm9/3’300) at 25oC using the mobile phase of acetonitrile-triethylamine acetate buffer (20mM and pH 8), which contains 10mM Nero vancomycin as chiral selector with a v/v % of 35%/65%. The effects of the type and the concentration of the chiral selector, the amount of acetonitrile and the buffer pH on the enantiomeric separation have been studied. The method has been examined regarding the linear range, the repeatability, the Detection limit and the quantification limit have been studied. The dynamical linear arrange of 1-50 Microgram per mL With the correlation of 0.9906 were found for R enantiomers and The dynamical linear arrange of 1-70 Microgram per mL With the correlation of 0.9939 were found for S enantiomers. The Accuracy of the method for 5 analyzers and measurements in a concentration of 10 micrograms per ml based on relative standard deviation and the under peak level was found to be 1.8 to 9.4 percent for each of R and S enantiomers and a detection limit of 0.6 micrograms per ml was found for R enantiomer and 0.4 microgram per ml for S enantiomer.