Room temperature biosynthesis and characterization of silver nanoparticles using Mango leaf extract

Currently, there is growing need to develop eco-friendly and body benign nanoparticle synthesis processes without use of toxic chemicals in the synthesis protocols to avoid adverse effects in biomedical applications and elsewhere. Biosynthesis of nanoparticles by plant extracts is currently more attractive than other biological systems because of their advantages such as their availability, rapid single-step method, low-cost, eco-friendly without using high pressure, energy or toxic chemicals, and safe for human therapeutic use [1].Herein, we are reporting the one step green synthesis of silver nanoparticles (AgNPs) at room temperature by using mango leaf extract as a reducer and stabilizer without addition of any other external reducing agent. The leaf extract of mango is known to possess a variety of phenolic compounds [2] that could probably mediated the reduction by donating electrons and can act as capping agent [3]. The surface plasmon resonance (SPR) peak intensity depends on pH, quantity of leaf extract, concentrations of AgNO3, reaction temperature and reaction time, hence the effect of these parameters were also studied to attend control over the particle size, and their stability. The synthesized AgNPs at optimum condition were characterized by means of UV-Vis, XRD, FT-IR and TEM. The absorption band centered at λmax 406 nm in UV-Vis reflects SPR of AgNPs. XRD analysis revealed that biosynthesized AgNPs are crystalline in nature. FT-IR analysis indicates that nanoparticles were capped with the constituent of leaf extract. TEM analysis suggested that the nanoparticles were spherical with 14 to 28 nm size with average size of 20.7 nm. It can be inferred from the study that fine tuning the bioprocess parameters will enhance possibilities of desired nano-product tailor made for particular applications.