Preparation and characterization of mixed-mode stationary phase in HPLC columns for the separation of complex samples

Traditional HPLC stationary phases often possess single interaction mechanism. Mixed-mode stationary phases are a kind of stationary phases in which multiple interactions exist between the stationary phases and analytes and at least two interactions should both contribute to the retention of the analytes [1]. Two column mixed mode stationary phase synthesized with a kind of stationary phase RPLC/HILIC in which two interactions exist between the stationary phase and analytes. In one of these groups are effective in isolation on a chain. In making this column in the first stage combines 3-amino propyl triethoxy silane to the silica attached and we at a later stage using lauric acid, acid chloride, the appropriate synthesis, and we stationary phase modified added and the hope and hydrocarbon chains along together and have been on a chain. The results of elemental analysis for this phase are C%: 10.02, N%: 1.26 and H%: 1.68. In the second column groups are affecting the two different chains. First Group dodecyl dimethyl chloro silane built into the surface of the silica attached and then Group 3-amino propyl triethoxy silane, both on the surface of silica placed and an amino group with the acid chloride prepared from formic acid amide group converted. The results of elemental analysis for this phase are C%: 6.67, N%: 0.53 and H%: 1.36. The as synthesized stationary phase was characterized by elemental analysis and Fourier Transform-Infrared Spectroscopy (FT-IR) [2]. In addition, the columns performance was compared with together in terms of column efficiency (N/m), retention factor (k) and resolution (Rs) [3].