Enrichment and determination of the trace amount of chlorpromazine based on solid phase microextraction using Al2O3 Nanoparticles in environmental and biological samples with the spectrophotometry method by chemometrics

Chlorpromazine(CPZ) is an antipsychotic phenothiazine which can be used in neurosis like schizoid ,dementia ,mania and is an auxiliary drug in curing high excitement and mental disorders, also it be applied as anti nauseam and curing the hiccup [1]. Many analytical methods including electrochemical[2] and instrumental[3] methods have been used for quantification of CPZ in variety media such as biological medium and pharmaceutical formulations, yet. Spectrophotometry has been widely used in the determination of pharmaceutical compounds [4]. In this study, enrichment and determination of trace amount of chlorpromazine (CPZ) in environmental and biological samples is presented based on solid phase microextraction using the nanoparticles of by Spectrophotometry of UV/Visible. This method is simple, fast, low cost, safe and available which compared with previous work easily can applied for determination sub-ppm amounts in everywhere. First, Al2O3 nano particles synthesized and then applied for extraction of CPZ. The effective parameters on the extraction efficiency of the drug such as type of organic solvent, pH donor phase optimized manually and the volume of aqueous phase, nanoparticle amount, extraction and desorption time, salt effect optimized by experimental design (Central Composite Design; CCD). Under the optimal conditions, limit of detection (LOD) were obtained. The linearity method was in the range 0.005-2 µg.mL-1 with correlation coefficient (R2) equals to 99.17 and pre-concentration factor acquired 366.53. Finally, this method was used for analysis environmental and urine samples with satisfactory results.