Optimized method for determination of an endocrine disrupter in water and milk samples by novel sorption-based on magnetic nanocomposite monolithic stir bar sorptive extraction followed by HPLC-UV

A simple method for the quantitative monitoring of an endocrine disrupter (bisphenol A (BPA)) in bottled mineral water and bottled milk samples is presented by stir bar sorptive extraction (SBSE) coupling to high performance liquid chromatography with ultra violet detection. The analyte were concentrated by SBSE based on magnetic nanocomposite monolithic (MNM) kit by combination of polyethersulfone (PES) solution and iron oxide nanopowder (nano Fe3O4) with certain rations. The size and morphology of synthesized kit was characterized using field emission scanning electron microscopy (FESEM) is equipped with energy dispersive X- Ray (EDX) spectroscopy system. Different parameters affecting SBSE extraction was carefully optimized, using experimental designs based on the central composite design (CCD). Limit of detection (LOD) of the BPA was 2.7×10-3 µg/mL for bottled mineral water sample and 7.8×10-3 µg/mL for bottled milk sample, whereas relative standard deviations (RSDs) were 6.73 % (n = 3, C = 0.02 µg/mL) for bottled mineral water sample and 8.49% (n = 3, C = 0.02 µg/L) for bottled milk sample. For bottled mineral water and bottled milk samples, good linearities were obtained for BPA with the correlation coefficients (R2) 0.9976 and 0.9992, respectively. The enrichment factors (EFs) of the method were 19.88 and 9.55 for bottled mineral water and bottled milk samples, respectively (theoretical enrichment factor was ~ 30). The proposed method was successfully applied to the analysis of bisphenol A (BPA) in bottled mineral water and bottled milk samples. Bisphenol A (BPA) are considered to be endocrine disrupters. The determination of the endocrine disrupters requires highly sensitive methods to evaluate all potential risks [1]. Since bisphenols (BPs) concentration in personal care products is suspected to be very low, a preconcentration technique should be required prior to their determination [2]. Stir bar sorptive extraction (SBSE) has become since its development in 1999 [3], one of the most popular preconcentration techniques due to its simplicity and robustness.