Chromatographic study of the interaction between Dichloromethane and Estrone (E1) by solid phase microextraction

Endocrine-disrupting chemicals are compounds that alter the normal functioning of the endocrine system [1]. Dichloromethane is widely used as an organic solvent and is found in paints, insecticides, degreasing and cleaning fluids, and other products. Dichloromethane receives an increasing consideration as potential endocrine disrupters. estrone (E1 (fluctuate with life stage, suggesting specific roles for its in biological and disease processes [2]. In this work, Solid Phase Micro Extraction fiber containing zirconium oxide/ graphene oxide nanosheets fiber was developed. The objectives of this study were to evaluate total, bound, and freely dissolved amount of the dichloromethane in the presence of estrone. The optimum conditions of microextraction process were selected and under optimized conditions, in vitro experiments were conducted utilizing a Head Space Solid Phase MicroExtraction (HS-SPME) combined with Gas Chromatography–flame ionization (FID) detector. Scatchard plot, binding constant, binding percentage, the number of binding sites per hormone molecule was determined. Binding percentage, the number of binding sites and binding constant were 63.16, 2.99and K=35.25×104respectively.