Fabrication of water-compatible superparamagnetic molecularly imprinted biopolymer for clean separation of baclofen from bio-fluid samples: A mild and green approach

Non-toxic and biocompatible Fe3O4 nanoparticles were employed as superparamagnetic core for imprinted biopolymer. Fe3O4 nanoparticles were used as core supporter to donate superparamagnetic properties; leading to quick and easy separation from sample solutions only by employment of external magnetic field.For the first time, a superparamagnetic molecularly imprinted biopolymer (SMIBP) was utilized as an eco-friendly and worth welting bio-sorbent at the service of dispersive solid phase extraction (d-SPE) procedure to provide a selective separation of baclofen form urine samples. This effective solid phase was biopolymer network with imprinted cavities grafted on the surface of Fe3O4 cores possessing an influential SiO2 layer. The prepared superparamagnetic MIBP was subsequently characterized by different techniques including SEM, VSM, FT-IR, and XRD, verifying high quality of the proposed solid phase. Under optimized conditions recommended by experimental design, developed SMIBP-d-SPE procedure supplied linear response over 1.0–2500.0 µg L−1 with a satisfactory detection limit close to 0.36 µg L-1, while repeatability concerned to intra-day and inter-day precision was presented as coefficients of variation lower than 3.9 %. These appropriate validation imply on high ability of proposed microextraction procedure for clean and effective enrichment of target analyte in complex human urine sample; consequently leading to accurate analyses at ultra-trace levels.