Effect of l-Arginine and Glycine on Thioacetamide induced liver damage in rats

Objectives: as some amino acids are precursors of many important biologic compounds essential for a normal functioning of body organism,L-Arginine has been reported to reduce organ injury and enhance liver regeneration by‌ experi-mentally modulating hepatic macrohaemodynamics. also Glycine has been shown to protect hepatocytes against hypoxia. activation of glycine-gated chloride channels using glycine treatment leads to blunt Kupffer cells activation and apoptosis of sinusoidal endothelial cells, in result, protecting against liver injury.glycine is a potent therapeutic immuno-nutrient for various kinds of chronic liver diseases.. based on this the present study was performed To evaluate the hepatoprotective effect of L-Arginine and glycine on thioacetamide (TAA) induced liver damage.Materials methods: In this experimental study, 40 male rats were divided into four groups of ten. Animals in the first group: (control) received water and normal food for rats, Group 2: (Disease control) received two injections of thioacetamide (200mg/kg, i.p.) at 24 hrs interval and the third group received both normal food and water and glycine was administered orally every 24hours(600mg/kg) for 14 days followed by two dose of thioacetamide,the fourth group received both normal food and water and l-arginie was administered orally every 24hours(500 mg/kg) for 14 days followed by two dose of thioacetamid. Blood and liver samples were taken afterwards, Serum ALT, AST, ALP and liver histological changes were assessed in every group of rats.Results Conclusion: in glycine and l-arginine treated groups ALT,AST and ALP enzymes showed significant decrease compared to disease control group and histopathological results showed less necrosis and fatty changes in hepathocytes compared to disease control groupe. the biochemical and histological results of our study indicated the protective effect of l-arginine and glycine against TAA induced liver damage in rats.