Evaluation of antiparasitic effect of ginger and glycyrrhiza extracts on neospora caninum (Nc) by embryonated chicken egg model

Objectives: Nc is a protozoan parasite from apicomplexan family which results abortion of fetus of various animals. Previous studies showed that embryonated chicken egg could be used as a successful model to study Nc. In this study effect of water extract of ginger and glycyrrhiza on Nc was evaluated using embryonated chicken eggs.Materials Method: Nc was cultivated on vero cell line as per standard procedure. Then tachyzoites were put in various culture media those are (1-only DMEM, 2-DMEM+ clindamycin, 3-DMEM+ ginger extract, 4-DMEM+ glycyrrhiza extract, and 5-DMEM without tachyzoite) and incubated at 37Ċ in Co2 incubator for 24 hrs. Then 25, 9 days old embryonated chicken eggs were divided into 5 groups each consisted of 5 eggs, eggs of each group received 2×105 tachyzoite of above mentioned media in chorioalantoic fluid. The inoculated eggs were kept in seter and daily inspected for embryo dead till 12 days post inoculation.Results Conclusion: Embryos of control group and the group received Nctreated with ginger extract were alive till day 12 post inoculation, while embryos of other groups showed various number of dead during inspection days. The difference related to dead of embryo among control and ginger treated groups with other groups was statistically significant. To our knowledge this is the first report related to use of embryonated chicken egg for evaluating effect of drug on Nc.