پديدآورندگان :
Elshiekh M Department of Physiology, Faculty of Medicine, University of Medical Sciences, International Campus, Tehran, Iran , Kadkhodaee M Department of Physiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran , Seifi B Department of Physiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran , Ranjbaran M Department of Physiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
چكيده فارسي :
Ischemia reperfusion (IR) injury, which is commonly seenin the field of renal surgery or transplantation, is a majorcause of acute renal failure (ARF). The aim of this study was to assess the combined effects of ischemic preconditioning (IPC) and recombinant human erythropoietin (EPO) on nitric oxide synthase in ischemia reperfusion rat model. Rats were subjected to 50 min bilateral ischemia followed by 24 h reperfusion. Animals in the examine group were administered EPO (5000 IU/kg, i.p) 30 min before ischemia, plus application of ischemic preconditioning (IPC) which was performed by three cycles of 3 min ischemia and 3 min reperfusion.Rats were classified as follows: 1) sham; 2) IR; 3) IPC + IR; 4) EPO + IR; 5) IPC + EPO + IR. Rats were sacrificed at 24 h after IR injury. Gene expression of iNOS and eNOS were assessed by RT-PCR. Treatment with IPC or EPO either alone or in combination increased gene expression of iNOS and eNOS compared to IR group. In addition, EPO + IPC and EPO treatment produced enchancediNOS gene expression compared to IPC group. Moreover, IPC and EPO + IPC showed more powerful effect on up regulation of eNOS gene expression compared to EPO group. These findings indicate that both of the treatment settings sharedsimilar mechanisms of up-regulating nitric oxide synthases. However, IPC combined with EPO had no synergistic effect on IR injury.
