پديدآورندگان :
Pilehchian Langroudi Reza r.pilehchian@rvsri.ac.ir Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, (AREEO), Alborz, Karaj, Iran , Pardis Ali Reza Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, (AREEO), Alborz, Karaj, Iran , Jabbari Ahmad Reza Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, (AREEO), Alborz, Karaj, Iran , Eskandari Abbas Ali Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, (AREEO), Alborz, Karaj, Iran
كليدواژه :
Clostridium , vaccine , enterotoxemia , ultrafiltration , system
چكيده فارسي :
Genus Clostridium, gram positive spore forming anaerobes are very important bacteria for human and animals pathogenesis. Clostridial diseases frequently occurs in Iranian farms and different Clostridium species are responsible for high mortality in domestic animals like as sheep and goats. The gole of this project was to produce Toxoid vaccine ageinest enterotoxemia disease in Iran. In the present study, three groups of experimental vaccines were produced and compared to each others. 1st group as bacterin tetravalent vaccine which produced in 20 liters glass vessels and adsorbed on aluminium hydroxide adjuvant. 2nd group as bacterin tetravalent vaccine produced within in 12 liters fermenter and adsorbed on aluminium hydroxide adjuvant. Finally, 3rd group as bacterin-toxoid tetravalent vaccine was produced in 12 liters fermenter and adsorbed on aluminium hydroxide adjuvant. Analysis for freedom from abnormal toxicity, safety and potency tests were performed for all produced vaccine groups. The results showed that the 3rd group of produced vaccine by fermenter and purified by ultrafiltration system is the best choice for scale up.
