The effect of gender and weight on drug interaction between phenylbutazone and thiopental on recovery times in rats

Objectives: Although new drugs are used in anesthesia today, barbiturates are used as an anesthetic drug in animal laboratory. As weight, sex and drug interactions are important factors in anesthesia, the aim of this study was to investigate the effects of gender and weight on clinical parameters in the thiopental anesthesia with phenybutazone drug interactions.Materials Methods: 40 females and 40 males of wistar rats with 180-200 g were randomly assigned into two groups (20 rats in each subgroup). Each subgroup of males and females in the diet (high calorie and low calorie) was different. They were divided into control and experimental groups in each subgroup of different diet. The experimental subgroup which was injected phenylbutazone 20% (20 mg/kg) 1 minutes before the injection of anesthetic thiopental 0.5% (30 mg/kg) and the control group was given normal saline. All injections were performed intraperitoneally to animals. Anesthetic clinical parameters including loss of righting reflex, abdominal reflex, pedal reflex, righting reflex, imbalance and walking normally in both induction and recovery phases were measured by a stopwatch.Results: The timing of recovery time in the experimental group compared to the control groups in two different sexes with different diet increased. In the control group of male and female with high calorie and low calorie, no significant difference was observed (P 0.05). While in the experimental groups of both sexes in some recovery parameters such as the abdominal reflex, pedal reflex, Righting and imbalance walking, there was a significant difference (P 0.05).Conclusion: The presence of phenybutazone leads to an increase in thiopental free form and in its distribution in tissues and consequently increases the recovery time. Significant differences in some parameters were observed between the experimental groups of high-calorie and low-calorie in recovery from the anesthesia but these differences are more marked in female.