Antifungal activity of denrosomal nanozattaria multiflora against dermatophytes strains

Objectives: The incidence of superficial dermatophytosis is high in domestic animals, and there remains a need for effective topical antifungals. Also, the increasing resistance to antifungal compounds and the reduced number of available drugs led us to search therapeutic alternatives among aromatic plants and their essential oils, empirically used by antifungal proprieties. In this study, we evaluated the in vitro antifungal susceptibility of dendrosomalnanozattaria against dermatophytes strains. Materials and methods: The MICs and minimum fungicidal concentrations (MFCs) against 50 clinical strains of Microsporum canis, Microsporum gypseum and Trichophytonm entagrophytes were determined using broth microdilution assay following the guidelines of the Clinical and Laboratory Standards Institute (CLSI) for filamentous fungi M38-A. CFU counts were performed over a period of 48 h of incubation. The minimum inhibitory concentration (MIC) was defined as the lowest concentration of extract that completely inhibited visible growth of microorganisms in the microdilution wells and the minimum fungicidal concentration (MFC) was determined by sub-culturing the negative wells on potato dextrose agar (PDA) plate and was the lowest concentration that yielded negative sub-cultures. Results and conclusion: The MIC range against the dermatophyte isolates tested was 0.25 to 2 µg/ml, with dendrosomalnanozattaria being fungicidal against 80% of the dermatophytes species. There was no increase in MIC for any strains following repeated exposure. Dendrosomalnanozattariademonstrated potent susceptibility against all dermatophytes tested, and none of the isolates within this test panel demonstrated the potential for the development of resistance. Thus, future clinical studies of denrosomalnanozattaria against dermatophytes may be warranted for the treatment of superficial dermatophytoses. The results of this research help to clinical veterinarians in the treatment of dermatophyte, so that they can choose the new and effective medication and prevent the failure of medical treatment.