پديدآورندگان :
Giorgi Mario mario.giorgi@unipi.it Department of Veterinary Clinics, University of Pisa, Italy , Vercelli Cristina Department of Veterinary Clinics, University of Pisa, Italy , De Vito Virginia Department of Veterinary Clinics, University of Pisa, Italy , Dezutto Daniela Department of Veterinary Clinics, University of Pisa, Italy , Bergagna Stefania Department of Veterinary Clinics, University of Pisa, Italy , Salvadori Marco Department of Veterinary Clinics, University of Pisa, Italy , Re Giovanni Department of Veterinary Clinics, University of Pisa, Italy
كليدواژه :
Bearded dragons , enrofloxacin , ciprofloxacin , pharmacokinetics , pharmacodynamics
چكيده فارسي :
Objective: Enrofloxacin (E) is commonly used in veterinary medicine. It is necessary to perform pharmacokinetic/dynamic studies to minimize the selection of resistant mutants of bacteria and extend the efficacy of antimicrobial agents.Materials Methods: Eight healthy adult Pogona vitticeps were assigned into two groups of equal size and treated with a single intramuscular injection of E at 10 mg/kg. Blood samples were withdrawn at different scheduled times for each group and rectal swabs were collected. E and ciprofloxacin (active metabolite) blood concentrations were quantified by an HPLC validated method, while the in vitro antimicrobial susceptibility was evaluated by the Kirby-Bauer disk diffusion susceptibility test.Results Conclusion: The pharmacokinetic profiles of E gave similar pharmacokinetic parameters irrespective of the collection time schedule. Bacteria isolation showed the presence of both E. coli, Salmonella enterica subspecies enterica and subspecies 3a, Proteus spp., and Pseudomonas spp. The majority of isolated colonies were sensitive to E, but the treatment did not reduce the number of bacteria in faeces. Results suggest that E is able to reach blood concentrations high enough to kill susceptible bacteria (MIC 0.9 µg/mL), but at the same time does not significantly affect intestinal bacteria.,
