پديدآورندگان :
Ashtarinezhad A Azadeh_Ashtarinezhad@yahoo.com Occupational Health Research Center, School of Public Health, Iran University of Medical Sciences, Tehran, Iran and Department of Occupational Health, School of Public Health, Iran University of Medical Sciences, Tehran, Iran , Panahyab A Young Researchers and Elite Club, Central Tehran Branch, Islamic Azad University, Tehran, Iran , Farnam G Department of Toxicology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran , Mohamadzadeh B Department of Toxicology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran , Shirazi Farshad H Department of Toxicology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran and Pharmaceutical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
كليدواژه :
Metronidazole , FTIR , MSP , teratogenicity , fetus , legs , arms
چكيده فارسي :
Objectives: Metronidazole as a derivative of nitromidazole is active against the anaerobic bacteria and protozoa. There is a relationship between using this drug and various birth defects suggesting teratogenicity for this drug when used by pregnant women. The purpose of this study was to investigate the teratogenic effects of Metronidazole during the growth of mice fetus legs and arms using the novel technique of FTIR spectroscopy.Materials Method: Pregnant mice were injected with metronidazole (60 mg/kg on gestation day 9), the fetuses were dissected on day 15 of gestation and then fixed by fixative solution. Tissue sections (10 µm) of control and metronidazole-treated legs arms were used for FTIR measurement in the wavenumber region of 4000-400 cm^-1.Results Conclusion: There were not any variations between control and treated legs but some shifts in bands from the spectra of treated hands were seen. An absorption band (at 1334 cm^-1) in control hand spectra coming from the stretching C-H was shifted to CH2 wagging. A band at 2918 cm^-1 in control leg tissue was divided to two bands in the fetus spectra of treated mothers. As the result of fetus FTIR spectra comparison between Metronidazole treated and control samples, only a mild teratogenic effects have been observed in the spectra originated from fetus hands. This is also the case from pharmacological studies. FTIR has proven its applicability for teratogenicity studies in this experiment.,
