پديدآورندگان :
Nafar Sefiddashti M Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran , Tahmasebian Ghahfarokhi N Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran , Sadeghi Sefiddashti M Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran , Ahmadi E eahmadihf@yahoo.com Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran , Hassanpour H Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran , Nazari H Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran
كليدواژه :
wild celery , sperm motility , H2O2 , oxidative stress , epididymal sperm
چكيده فارسي :
Objectives: Spermatozoa are susceptible to oxidative stress due to the high membrane content of polyunsaturated fatty acid and the intracellular deficiency of antioxidant enzymes. Kelussia odoratissima Mozzaf (wild celery; mountain celery) is a medicinal herb that belongs to the family of Umbelliferae and grows at high altitudes (more than 2000 m). This self-growing plant is exclusively found in Iran. Antioxidant potential of Kelussia odoratissima Mozzaf is comparable to α-tocopherol and butylatedhydroxytoluene when assayed by various methods. The aim of this study was to evaluate the effects of aqueous extract of Kelussia odoratissima Mozzaf on ovine epididymal sperm exposed to in vitro oxidative stress. Materials Methods: Testes of sexually mature rams were collected in local abattoir and transported to the laboratory, where epididymal sperms were expelled from caudal epididymis into HEPES buffered TCM199. Sperms were incubated at 37˚C with 100 µM H2O2 or 100 µM H2O2 + 0.01% (w/v) wild celery extract and no treatment (control) in HEPES buffered TCM199 at concentration of 10×106 sperm/ml for 30 min. Motility of fresh sperm and incubated sperms were analyzed by computer-assisted sperm analysis system (CASA). Each treatment was replicated 5 times anddata of sperm motility parameters were analyzed by One-way ANOVA using IBM SPSS 20. Differences at a level of P 0.05 were considered significant. Results Conclusion: Incubation of sperms with H2O2 caused significant decrease in sperm motility and progressive motility (42.8±1.74 and 32.6±3.16% respectively) compared with control (74±4.15 and 68.4±6.60%) and fresh groups (78.2±1.77% and 73.7±1.78%). Co-incubation of sperms with H2O2 and extract significantly prevented this effect (62.6±2.40% and 51.5±1.98%). This study showed that aqueous extract of Kelussia odoratissima Mozzafcan prevent detrimental effects of oxidative stress on ovine epididymal sperms.,
