Oleuropein pretreatment improves anaesthesia induction and sleep times in rats

Objective: The aim of the present study was to evaluate oleuropein effects on induction and sleep times mediated by anaesthetic drugs in rats. Materials and Methods: Sprague–Dawley rats were divided as follows: Propofol, Propofol-Ketamine (Pro.-Ket.), Xylazine-Ketamine (Xyl.-Ket.), and 3 oleuropein pretreated groups (Ole.-Pro., Ole.-Pro.-Ket. and Ole.-Xyl.-Ket.). The oleuropein pretreated-groups received oleuropein (15 mg/kg bw, orally) for 10 consecutive days. Propofol 100 mg/kg, xylazine 3 mg/kg and ketamine 75 mg/kg once as ip was used in the 11th day of the treatment. Induction and sleep times of anaesthesia were measured by checking lip, tail and pedal pinch tests, as visually. Results and Conclusion: Oleuropein pretreatment significantly increased sleep time in Ole.-Pro. group in comparison with Pro.-Ket. and Ole.-Pro.-Ket.-treated rats (p 0.01). Although Pro.-Ket. and Ole.-Pro. groups indicated higher induction times versus other groups, these enhancements were not statistically significant (p 0.05). We confirmed that propofol is a promising anaesthetic drug and oleuropein pretreatment improves sleep time in anaesthetic rats, which its antioxidant effects have recently been examined in our laboratory.