Screening of some Iranian bentonite to adsorb Aflatoxin B1 and OchratoxinA in rumen fluid

Objectives: Mycotoxins are the most prevalent toxins usually found in animal feed and caused to acute and chronic toxicities in animals and humans. The main strategy for reducing the exposure to mycotoxins is decrease their availability bymycotoxin-adsorbing agents like bentonite. Therefore, this study was conducted to evaluate the efficacy of Iranian bentonite to remove AFB1 and OTA.Materials Methods:15bentonite samples wereobtained from the mines of South-Khorasan (8 samples), Fars (3 samples) and Isfahan (4 samples) provinces. In addition, commercial toxin binder Mycofix ®plus was used as control. Briefly, 20% rumen fluid solution were prepared andsupplemented with AFB1 (1000 ng.L-1) or OTA (1000 ng. mL-1). Subsequently, 5 mg bentonite was added to toxin-supplemented rumen fluid. The rates AFB1 and OTA to bentonite was 1:500. The toxin-adsorption activities were determined after 2 h incubation on an incubator shaker at 39°C and 100 rpm in darkness. The toxin-content was extracted three times with chloroform and analyzed by High Performance Thin Layer Chromatography (HPTLC). The experiment was carried out in randomized complete block (RCB) design with three replications.Results Conclusion: Theresults on AFB1 showed that two samples from South-Khorasan province could completely remove AFB1 from rumen fluid and showed more AFB1-adsorbtion activity than Mycofix ® plus. In addition, other sample from South-Khorasan, two samples from Esfahan and 1 sample from Fars could considerably adsorb AFB1 by 90-97%. Furthermore, 4 samples couldn’t remarkably adsorb AFB1. The results on OTA showed that none of the bentonite samples as well as Mycofix ® plus could significantly adsorb OTA from rumen fluid. In conclusion, some Iranian bentonites from South-Khorasan province suggest to reduce AFB1 risk in animal feed. However, the future investigation to find a promising candidate for reduce risk of OTA in animal feed is necessary.,