Effect of soy meal on histomorphometery of duodenal villi and serum levels of calcium and phosphorus in the blood of surri mice Balb/c

Objectives: Some benefits and lacking side effects in soy bean consumption have been caused being presented as a cheap protein source in food industry. The objective of this study is to evaluate the effect of soy meal on duodenum and serum levels of calcium and phosphorus.Materials Methods: 30 female surri mice after fostering period were selected and after adaptation with the environment, on the basis of two kinds of diets, were divided into two groups each with ten individuals. The control group was fed on a diet of complete protein without soy bean and the treatment group received a diet containing 20% soy bean. After three months the mice were anesthetized, the hearts were removed and blood samples for determination of the serum levels of calcium and phosphorus were taken. After euthanizing and histological studies, provided photomicrographs were used for histomorphometric study by Axiovision software and the widths of top, middle and base of the villi were measured. Finally the data were analyzed using One-way analysis of variance and the significant level was considered as P 0/05.Results Conclusion: In histomorphometric study, it was observed that in the width of the top of villi there were no significant changes whereas the widths of the middle and base of villi there were significant decrease compared with the control group (P 0/05). Besides, the level of calcium in the group with soy bean consumption (6.48±0.30) compared with the control group (3.73±2.92) showed a significant increase (P 0/05). But the level of calcium in the group with soy bean consumption (11.56±4.71) did not show any significant change compared with the control group (11.66±4.03).It could be deduced that long term consumption of soy bean due to making changes in villi and also on the serum levels of calcium, might be resulted in malabsorption.