تعيين مقدار كمي بتاهيستين هيدروكلرايد در قرص هاي موجود در بازار با استفاده از روش كروماتوگرافي مايع با كارايي بالا

QUANTITATIVE DETERMINATION OF BETAHISTINE HYDROCHLORIDE IN COMMERCIAL TABLETS USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

A simple, rapid, precise and accurate isocratic reversed-phase HPLC method was developed and validated for determination of Betahistine Hydrochloride in commercial tablets. Separation was achieved on a Phenomenax C18 column (150 mm × 4.6 mm, 5 µm) using a mobile phase consisting of acetonitrile-sodium acetate buffer (pH 4.7) (35:65, v/v) at a flow rate of 1.5 ml min-1 and UV detection at 254 nm. The column temperature was maintained at 40 ºC. The linearity of the proposed method was investigated in the range of 0.256-0.384 mg mL-1 (r = 0.9984). Precision were less than 2.0% and accuracy was between 99.81% and 100.88%. This method was validated and successfully applied in analysis of tablet dosage form because no chromatographic interferences from formulation excipients were found.

A simple, rapid, precise and accurate isocratic reversed-phase HPLC method was developed and validated for determination of Betahistine Hydrochloride in commercial tablets. Separation was achieved on a Phenomenax C18 column (150 mm × 4.6 mm, 5 µm) using a mobile phase consisting of acetonitrile-sodium acetate buffer (pH 4.7) (35:65, v/v) at a flow rate of 1.5 ml min-1 and UV detection at 254 nm. The column temperature was maintained at 40 ºC. The linearity of the proposed method was investigated in the range of 0.256-0.384 mg mL-1 (r = 0.9984). Precision were less than 2.0% and accuracy was between 99.81% and 100.88%. This method was validated and successfully applied in analysis of tablet dosage form because no chromatographic interferences from formulation excipients were found.