Sonographic .Evaluation of the Frozen Allograft Tendon Impregnated with the Mesenchymal Cells PRP in Lamb

Objectives: In this study the efficacy of the Mesechyamal cells, for binding between frozen allograft tendons qualitatively was investigated. Design: In the present study the type of the design was experimental and analytical study Animals: 15 four months lambs were selected for removal the both forelimbs SDF and regrafted using frozen allograft tendon of the same age. Procedures: The under strict aseptic condition mid portion of SDF of both forelimbs were removed and was grafted with allografts harvested from slaughter house and frozen under 20 degrees C0 for 60 days in all the 15 lambs, which were subsequences for grafted immediately after collections. Subsequently they were divided into 3 subgroups of 5 lambs each. The first group was acted as control ,whereas the second group 1 ml PRP was injected at the site of anastomosis and in the third group 1 ml of mesenchymal cells ( 10 6) was used at the site of grafting.CSA ,width, thickness echogenicity score, fiber score,peritendinous edam and inflammation,peritendinous fibrous tissue formation, calcification of tendon, tendon degeneration and tendon union were parameters evaluated at normal ,after grafting and on 60 days . Results: The grafting were done under satisfactory anesthesia .in control group there were significant changes in the CSA,width,thickness,echogenicity score and fiber score and sporadic changes in peritendinous edema and with inflammation, but despite of recorded changes in above parameters in second and third groups, there were no significant changes. Conclusion and Clinical Relevance: The results of this study showed that Mesenchymal cells PRP can besides enhancing the content of the healing tissue at the site of grafting ,it also help for early condensation of fibrous tissue the site of anastomosis. Sonographic evaluation is quite reliable for early normalization of grafted tendon as far as early weight bearing is concerned in the lamb