تعيين اثر نانوذره اكسيد روي بر اتصال اوليه و ميزان بيان ژن fimH در UPEC

Effects of Zno nanoparticles on initial adhesion and fimH gene expression level of Uropathogenic Eschercia coli

اشريشيا كلي يوروپاتوژن (UPEC) عامل بخش عمده عفونت هاي ادراري (UTI) است. يكي از مراحل اوليه و اصلي در روند عفونت زايي UPEC اتصال است. يكي از ساختارهاي سطحي باكتري كه در اتصال نقش مهمي دارد فيمبريه تيپ 1 است. هدف اين مطالعه بررسي اثر نانوذره اكسيد روي (ZnO) بر اتصال اوليه و ميزان بيان ژن fim H در UPEC مي باشد. 4 ايزوله UPEC مورد بررسي قرار گرفت. بيوفيلم هر 4 ايزوله تحت تاثير غلظت sub MIC از نانوذره اكسيد روي (g/mlµ1250) قرار گرفت. سپس ميزان بيان ژن fim H با روش Real-Time PCR ارزيابي شد. ميزان بيان در هر 4 ايزوله كاهش پيدا كرد. كمترين و بيشترين ميزان كاهش بيان به ترتيب 1.4 و 16.37 بود. نتايج بيان ژن با نتايج فنوتيپي هم خواني داشت.

Uropathogenic Escherichia coli (UPEC) strains are the main causes of urinary tract infections. Adhesion is one of the main and primary steps of UPEC pathogenicity. Type 1 fimbriae is one of the bacterial surface structures that plays an important role in bacterial adhesion. The aim of this study was to evaluate effects of ZnO nanoparticles on the initial adhesion and fimH gene expression level of UPEC. Four UPEC isolates were used in this study. All isolates were exposed to sub-minimum inhibitory concentration of ZnO nanoparticles (1250 μg/ml). Expression of the fimH gene was evaluated by Real-time PCR. According to the results, presence of nanoparticles reduced the fimH expression level in all four isolates. The highest and lowest rates of down-expression were 1.4-fold and 16.37-fold, respectively. Moreover, these results were consistent with phenotypic observations. However, it is recommended to conduct further studies on gene expression and bacterial adhesion to surfaces to prove whether ZnO nanoparticles could completely prevent UPEC adhesion.