Measurement of catalase activity in human lung epithelial adenocarcinoma cell line (A549), treated with B2R peptide

Cells through antioxidant enzymes such as superoxide dismutase (SOD), catalase and glutathione peroxidase prevent or repair the damages caused by reactive oxygen species. Catalase converts the hydrogen peroxide to the harmless product water. There are several methods for measuring the activity of antioxidant enzymes in cultured cells, such as assay enzyme activity on gels, or western blots. In this study, we measured the activity of catalase in A549 cell line treated by various concentrations of B2R (6, 9, 12, 15, and 25 µg/ml), through studying the changes in H2O2 absorption by UV/visible spectrophotometer. Results demonstrated that catalase has its most activity in treated cells at 12 µg/ml of B2R for 24 h. The cytotoxic effect of B2R peptide was determined by MTT assays. Results shown that there is linear correlation between various concentrations of B2R peptide and cytotoxicity effect of peptide on A549 cultured cells.