ﻣﻄﺎﻟﻌﻪ آﻧﺎﺗﻮﻣﯽ و ﻫﯿﺴﺘﻮﻣﻮرﻓﻮﻣﺘﺮي ﺑﺨﺶ ﺧﻮن ﺳﺎز ﮐﻠﯿﻪ ي ﻣﺎﻫﯽ ﺑﺎرﺑﻮس ﭘﮑﺘﻮراﻟﯿﺲ(ﺑﺮزم )

Anatomical and Histomorphometrical study of hematopoietic portion of kidney inm

ﻣﺎﻫﯽ ﺑﺎرﺑﻮس ﭘﮑﺘﻮراﻟﯿﺲ ﯾﺎ ﺑﺮزم از ﺧﺎﻧﻮاده ﮐﭙﻮرﻣﺎﻫﯿﺎن در رودﺧﺎﻧﻪ ﮐﺎرون ﻣﯽ ﺑﺎﺷﺪ. ﮐﻠﯿﻪ ﻣﻬﺮه داران ﯾﮑﯽ از اﻧﺪام ﻫﺎي ﻣﻬﻢ ﺑﺪن ﻣﯽ ﺑﺎﺷﺪ ﮐﻪ ﻫﻤﻮﺳﺘﺎز ﻣﺎﯾﻌﺎت ﺑﺪن را ﺗﻨﻈﯿﻢ ﻣﯽ ﮐﻨﺪ. در اﯾﻦ ﭘﮋوﻫﺶ ﺳﺎﺧﺘﺎر ﺑﺎﻓﺖ ﺷﻨﺎﺳﯽ ﮐﻠﯿﻪ10 ﻗﻄﻌﻪ ﻣﺎﻫﯽ ﺑﺮزم ﻣﻮرد ﺑﺮرﺳﯽ ﻣﺎﮐﺮوﺳﮑﻮﭘﯿﮏ و ﻣﯿﮑﺮوﺳﮑﻮﭘﯿﮏ ﻗﺮار ﮔﺮﻓﺘﻨﺪ. ﭘﺲ از ﺧﺎرج ﺳﺎﺧﺘﻦ ﮐﻠﯿﻪﻫﺎ از ﻣﺤﻮﻃﻪ ﺑﻄﻨﯽ، از ﻗﺴﻤﺖﻫﺎي ﻣﺨﺘﻠﻒ ﮐﻠﯿﻪ ﻧﻤﻮﻧﻪﺑﺮداري اﻧﺠﺎم ﮔﺮﻓﺖ و ﺟﻬﺖ اﻧﺠﺎم ﻋﻤﻞ ﺛﺒﻮت، اﯾﻦ ﺑﺎﻓﺖﻫﺎ در ﻣﺤﻠﻮل ﺑﻮﺋﻦ ﻗﺮار داده ﺷﺪﻧﺪ، ﺳﭙﺲ ﺑﻪ روش ﻣﻌﻤﻮل ﺗﻬﯿﻪ ﻣﻘﺎﻃﻊ ﺑﺎﻓﺘﯽ، ﺑﺮشﻫﺎﯾﯽ ﺑﻪ ﺿﺨﺎﻣﺖ 6-5 ميكرومتر تهيه -5 ﻣﯿﮑﺮوﻣﺘﺮ ﺗﻬﯿﻪ و ﻣﻮرد رﻧﮓآﻣﯿﺰي ﻗﺮار داده ﺷــﺪﻧﺪ. ﻣﻄﺎﻟﻌﺎت ﻣﺎﮐﺮوﺳــﮑﻮﭘﯿﮏ ﻧﺸــﺎن داد ﮐﻪ ﮐﻠﯿﻪ ﻣﺎﻫﯽ ﺑﺮزم از ﻧﻈﺮ ﻣﻮﻗﻌﯿﺖ آﻧﺎﺗﻮﻣﯿﮑﯽ در زﯾﺮ ﺳﺘﻮن ﻓﻘﺮات ﻗﺮار دارد و ﻣﺸﺘﻤﻞ ﺑﺮ ﺳﻪ ﺑﺨﺶ ﺳﺮ، ﺑﺪﻧﻪ و دم ﻣﯽﺑﺎﺷﺪ. ﻫﻤﭽﻨﯿﻦ ﻣﺸﺎﻫﺪات ﻣﯿﮑﺮوﺳﮑﻮﭘﯽ ﻧﺸﺎن داد ﮐﻪ ﮐﻠﯿﻪ ﻣﺎﻫﯽ ﺑﺮزم ﻫﻤﺎﻧﻨﺪ ﺳﺎﯾﺮ ﻣﺎﻫﯿﺎن ا ﺳﺘﺨﻮاﻧﯽ، از دو ﺑﺨﺶ ﺧﻮن ﺳﺎز و ﻟﻨﻔﻮﺋﯿﺪي و ﺑﺨﺶ دﻓﻌﯽ ﺗ ﺸﮑﯿﻞ ﮔﺮدﯾﺪه ا ﺳﺖ. ﻗ ﺴﻤﺖ ﺳﺮ ﮐﻠﯿﻪ اﯾﻦ ﻣﺎﻫﯽ ﻧ ﺴﺒﺖ ﺑﻪ ﻗ ﺴﻤﺖ ﺑﺪﻧﻪ داراي ﺑﺎﻓﺖﻫﺎي ﺧﻮﻧ ﺴﺎز و ﻟﻨﻔﻮﺋﯿﺪي ﺑﯿ ﺸﺘﺮي ﻣﯽﺑﺎ ﺷﺪ. در ﻣﻄﺎﻟﻌﻪ ﻣﯿﮑﺮو ﺳﮑﻮﭘﯽ ﺑﺎﻓﺖﻫﺎي ﺧﻮن ﺳﺎز و ﻟﻨﻔﻮﺋﯿﺪي ﺑﻪ و ﺿﻮح ﻣﺮاﺣﻞ ر ﺷﺪ و ﺗﮑﺎﻣﻞ ﮔﻠﺒﻮلﻫﺎي ﻗﺮﻣﺰ و ﺳﻠﻮلﻫﺎي ﻟﻨﻔﻮﺋﯿﺪي ﻣﺸﺎﻫﺪه ﺷﺪﻧﺪ. ﻻﺑﻼي ﺳﻠﻮلﻫﺎي ﺧﻮﻧﺴﺎز ﮐﻠﯿﻪ، ﺳﻠﻮلﻫﺎي ﻣﻼﻧﻮﻣﺎﮐﺮوﻓﺎژ ﻧﯿﺰ ﻣﺸﺎﻫﺪه ﮔﺮدﯾﺪﻧﺪ. ﺗﻌﺪاد ﺟ ﺴﻤﮏ ﻫﺎي ﮐﻠﯿﻮي در ﺑﺨﺶ دﻓﻌﯽ در ﺑﺨﺶ ﺑﺪﻧﻪ(11/9±2/94)، در ﺑﺨﺶ ﺳﺮ ﮐﻠﯿﻪ (2/9±1/5) ﮐﻪ ﺗﻌﺪاد آﻧﻬﺎ در ﺑﺨﺶ ﺑﺪﻧﻪ ﺑﯿﺸﺘﺮ از ﺳﺮ ﮐﻠﯿﻪ ﻣﯽ ﺑﺎﺷﺪ و ﺑﻄﻮر ﺗﻘﺮﯾﺒﯽ ﻣﯽ ﺗﻮان ﮔﻔﺖ ﮐﻪ 2 ﺑﺮاﺑﺮ اﯾﻦ ﻣﯿﺰان در ﺑﺨﺶ ﺳﺮ ﮐﻠﯿﻪ اﺳﺖ.

Barbus pectoralis or berzem is cyprinidae inhabit Karoon River. The kidney of the vertebrate is the main organ which balances the body fluid homeostasis. At present study, kidneys of 10 normal Barbus pectoralis have been studied macroscopically and microscopically. The kidneys were collected by autopsy and tissue samples were sectioned by routine paraffin embedding and stain by H&E and PAS. Macroscopic observation showed that the kidney in Barbus Pectoralis situated in ventral region of vertebral column. The kidney can be divided to; head, body and tail portion according to their relative position. Microscopic studies showed that the kidneys consisted of excretory portion and hematopoietic-lymphoid tissues. Hematopoietic-lymphoid tissue consisted of cells which are found in different stages of development to blood cells. Cells with melanin pigment were seen in hematopoietic tissue; these cells called melanomacrophages which organized in melanomacrophage centers. The number of renal corpuscles in body portion (11/2±9/94) is nearly two times more than head portion (2/1±9/5).