ﺟﺪاﺳﺎزي و ﺷﻨﺎﺳﺎﯾﯽ ﻣﻮﻟﮑﻮﻟﯽ ﻻﮐﺘﻮ ﺑﺎﺳﯿﻞ ﮐﺎزﺋﯽ از روده ﻣﺎﻫﯽ ﮐﭙﻮر ﻧﻘﺮه اي

Isolation and identification of the lactobacillus casei from the intestinal Silver carp (Hypophthalmichthys molitrix)

ﻻﮐﺘﻮ ﺑﺎ ﺳﯿﻞ ﻫﺎ از ﻣﻬﻤﺘﺮﯾﻦ ﻣﯿﮑﺮواورﮔﺎﻧﯿ ﺴﻢ ﻫﺎي ﭘﺮوﺑﯿﻮﺗﯿﮏ ﺑﻮده. ﻻﮐﺘﻮ ﺑﺎ ﺳﯿﻞ ﻫﺎ، ﺑﺎ ﺳﯿﻞ ﮔﺮم ﻣﺜﺒﺖ، ﺑﺪون ﺣﺮﮐﺖ، ﺑﺪون اﺳــﭙﻮر وﮐﺎﺗﺎﻻز ﻣﻨﻔﯽ ﻫﺴــﺘﻨﺪ؛ و ﻗﻨﺪ ﻫﺎي ﻣﺨﺘﻠﻒ را ﺑﻪ ﻻﮐﺘﺎت و اﺳــﺘﺎت ﺗﺒﺪﯾﻞ ﻣﯽ ﮐﻨﻨﺪ ﻫﻤﭽﻨﯿﻦ ﻻﮐﺘﻮﺑﺎﺳــﯿﻠﻮس ﻫﺎ داراي اﺛﺮ آﻧﺘﺎﮔﻮﻧﯿ ﺴﺘﯽ ﺧﻮﺑﯽ ﺑﺮ ا ﺳﺘﺎﻓﯿﻠﻮﮐﻮك اورﺋﻮس ، ا ﺷﺮﯾ ﺸﯿﺎ ﮐﻠﯽ و آﺋﺮوﻣﻮﻧﺎس ﻫﯿﺪروﻓﯿﻼ ﻫ ﺴﺘﻨﺪ. ﭘﺮوﺑﯿﻮﺗﯿﮑﻬﺎ ﺑﺎ ا ﺳﺘﻘﺮاردر ﻣﺤﯿﻂ روده ﻣﯽ ﺗﻮاﻧﻨﺪ ﺗﻌﺎدل ﻣﯿﮑﺮوﺑﯽ رادرﺟﻬﺖ ﺳﻼﻣﺖ و ﺳﻮدﻣﻨﺪي آﻧﻬﺎ اﻓﺰاﯾﺶ دﻫﻨﺪ. ﻋﻼوه ﺑﺮ اﯾﻦ ﭘﺮوﺑﯿﻮﺗﯿﮏﻫﺎ ﺑﺎ ﺳﻨﺘﺰ ﺑﺮﺧﯽ ﻣﻮاد ﺿﺮوري، ﻧﻘﺶ ﻣﻬﻤﯽ در ﺣﻔﻆ ﺳﻼﻣﺘﯽ ﻓﺮد ﻣﺼﺮف ﮐﻨﻨﺪه اﯾﻔﺎ ﻣﯽ ﮐﻨﻨﺪ. ﺑﺮاي ﺷﻨﺎﺳﺎﯾﯽ ﺑﺎﮐﺘﺮي از روش ﻣﻮﻟﮑﻮﻟﯽ اﻧﺠﺎم ﺷﺪ ﮐﻪ ﺑﺎ اﺳﺘﻔﺎده از آﻏﺎز ﮔﺮ ﻫﺎي ﻃﺮاﺣﯽ ﺷﺪه از ژن 16S rRNA ﺑﺮاي اﻧﺠﺎم واﮐﻨﺶ PCR، ﺑﺮاي ﺗﺸﺨﯿﺺ ﺟﻨﺲ ﻫﺎي ﻻﮐﺘﻮ ﺑﺎﺳﯿﻞ ﻫﺎ، ﻣﻮرد اﺳﺘﻔﺎده ﻗﺮار ﻣﯽ ﮔﯿﺮد. در اﯾﻦ ﺗﺤﻘﯿﻖ از 50 ﻣﺎﻫﯽ ﻧﻤﻮﻧﻪ ﺑﺮداري ازروده ﺻﻮرت ﮔﺮﻓﺖ ﮐﻪ در اﯾﻦ ﺑﻪ ﻣﯿﺰان 28 ﻧﻤﻮﻧﻪ ﻣﺜﺒﺖ ﻻﮐﺘﻮ ﺑﺎﺳﯿﻠﻮس ﮐﺎزﺋﯽ ﺑﻌﺪ از رﻧﮓ آﻣﯿﺰي و ﺗﺴﺖ PCRﺷﻨﺎﺳﺎﯾﯽ و ﺟﺪا ﮔﺮدﯾﺪ.

lactobacilli are the most important probiotic microorganisms, which Lactose bacilli are gram positive, without movement, without spores and catalase, and convert different sugars to lactate and acetate Lactobacillus also has a good antagonistic effect on Staphylococcus aureus, Escherichia coli an‎d Aeromonas Hydrophyla Probiotics that grow in the intestinal environment can increase the microbial balance for their health and well-being. In addition, probiotics, with the synthesis of some essential nutrients, play an important role in maintaining the health of the consumer To identify the bacterium, a molecular technique was used to detect the sex of lactobacillus using a primer designed from the 16S rRNA gene to perform a PCR reaction In this study, 50 fish were sampled The results of this study showed that 28 positive samples of Lactobacillus casei were identified and isolated after staining and PCR testing.