ﻣﻄﺎﻟﻌﻪ اﺛﺮ ﻣﻮﻧﺖ ﻣﻮرﯾﻠﻮﻧﯿﺖ (MMT) در ﺟﯿﺮه ﺑﺮ ﺷﺎﺧﺺ ﻫﺎي رﺷﺪ و ﺑﺎﻓﺖ ﺷﻨﺎﺳﯽ روده ﻗﺰل آﻻي رنگين كمان ( Oncorhynchus mykiss)

Study of dietary montmorillonite (MMT) on growth parameters and intestinal morphology of Rainbow trout (Oncorhynchus mykiss)

در اﯾﻦ ﻣﻄﺎﻟﻌﻪ اﺛﺮ ﻣﻮﻧﺖ ﻣﻮرﯾﻠﻮﻧﯿﺖ ﺟﯿﺮه ﺑﺮ ﺷﺎﺧﺺﻫﺎي ر ﺷﺪ اﻧﺪازهﮔﯿﺮي و ﻣﻘﺎﯾﺴﻪ ﺑﺎﻓﺖ ﺷﻨﺎ ﺳﯽ اﭘﯿﺘﻠﯿﻮم روده، در ﻣﺎﻫﯽ ﻗﺰل آﻻي رﻧﮕﯿﻦﮐﻤﺎن درﯾﮑﯽ از ﻣﺮاﮐﺰ ﭘﺮورش ﻣﺎﻫﯽ ﺳﺮد آﺑﯽ ﻣﻮرد ﺑﺮرﺳﯽ ﻗﺮار ﮔﺮﻓﺖ. ﻣﺎﻫﯿﺎن ﺑﻪ ﻣﺪت 7 روز ﺑﺎ ﺟﯿﺮه ﻏﺬاﯾﯽ ﭘﺎﯾﻪ و ﺗﺠﺎري ﺗﻐﺬﯾﻪﺷﺪه و ﺑﺎ ﺷﺮاﯾﻂ ﺳﯿﺴﺘﻢ ﭘﺮورﺷﯽ ﺳﺎزﮔﺎر ﺷﺪﻧﺪ. ﭘﺲ از اﺗﻤﺎم دورهي ﺳﺎزﮔﺎري، ﻣﺎﻫﯿﺎﻧﯽ ﺑﺎ ﻇﺎﻫﺮ ﺳﺎﻟﻢ (ﻋﺪم وﺟﻮد ﺑﺪ ﺷﮑﻠﯽ، ﺧﻮردﮔﯽ ﺑﺎﻟﻪ و ﻏﯿﺮه) و ﺑﺎ ﻣﯿﺎﻧﮕﯿﻦ وزﻧﯽ 20 ﮔﺮم ﺟﻬﺖ ﺷﺮوع دوره ي آزﻣﺎﯾﺶ اﻧﺘﺨﺎب ﺷﺪﻧﺪ. ﺗﻮزﯾﻊ ﺑﭽﻪ ﻣﺎﻫﯿﺎن ﺑﻪ ﻧﺤﻮي اﻧﺠﺎم ﺷﺪ ﮐﻪ ﻫﯿﭻﮔﻮﻧﻪ اﺧﺘﻼف ﻣﻌﻨﯽ داري ازﻟﺤﺎظ ﺑﯿﻮﻣﺲ ﺑﯿﻦ ﺗﯿﻤﺎرﻫﺎ وﺟﻮد ﻧﺪاﺷﺖ. اﯾﻦ آزﻣﺎﯾﺶ در ﻗﺎﻟﺐ ﯾﮏ ﻃﺮح ﮐﺎﻣﻼً ﺗﺼﺎدﻓﯽ ﺑﺎ ﺳﻪ ﺗﯿﻤﺎر و ﺳﻪ ﺗﮑﺮار ﺑﺮاي ﻫﺮ ﺗﯿﻤﺎر اﺟﺮا ﺷﺪ. ﺟﯿﺮهﻫﺎي ﻏﺬاﯾﯽ ﺷﺎﻣﻞ ﺳﻪ ﺗﯿﻤﺎر آزﻣﺎﯾ ﺸﯽ ﺑﻮد. ﯾﮏ ﺟﯿﺮه ﺑﻪﻋﻨﻮان ﺷﺎﻫﺪ (ﺟﯿﺮه ي ﺷـﻤﺎره 1 ) ﻓﺎﻗﺪ ﭘﻮدر ﻣﻮﻧﺖ ﻣﻮرﯾﻠﻮﻧﯿﺖ و 2 ﺟﯿﺮه ﺣﺎوي ﭘﻮدر ﻣﻮﻧﺖ ﻣﻮرﯾﻠﻮﻧﯿﺖ در ﻣﻘﺎدﯾﺮ20 و 40 گرم ﺑﺮ ﮐﯿﻠﻮﮔﺮم ﻏﺬا در ﻧﻈﺮ ﮔﺮﻓﺘﻪﺷﺪ. ﭘﺲ از 8 ﻫﻔﺘﻪ ﺗﻐﺬﯾﻪ، ﻣﺎﻫﯿﺎن ازآبﮔﺮﻓﺘﻪ ﺷﺪﻧﺪ. زﯾﺴﺖﺳﻨﺠﯽ و آزﻣﺎﯾﺶﻫﺎي ﻻزم ﺑﺮ روي آنﻫﺎ اﻧﺠﺎم ﮔﺮﻓﺖ. ﻧﺘﺎﯾﺞ ﺣﺎﺻﻞ ﻧﺸﺎن ﮔﺮوه ﻣﺎﻫﯿﺎن ﺗﻐﺬﯾﻪﺷﺪه ﺑﺎ 20 ﮔﺮم ﺑﺮ ﮐﯿﻠﻮﮔﺮم ﻣﻮﻧﺖ ﻣﻮرﯾﻠﻮﻧﯿﺖ داراي ﺑﯿﺸﺘﺮﯾﻦ ﻣﯿﺰان اﻓﺰاﯾﺶ ﻃﻮل و وزن ﺑﻮدﻧﺪ و اﺧﺘﻼف ﻣﻌﻨﯽ داري ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﮐﻨﺘﺮل داﺷﺘﻨﺪ. ﻟﯿﮑﻦ ﻫﯿﭻﮔﻮﻧﻪ اﺧﺘﻼف ﻣﻌﻨﯽ داري در ﺿﺮﯾﺐ ر ﺷﺪ وﯾﮋه و ﺿﺮﯾﺐ ﺗﺒﺪﯾﻞ ﻏﺬاﯾﯽ در ﺑﯿﻦ ﺗﯿﻤﺎرﻫﺎ ﻣﺸﺎﻫﺪه ﻧ ﺸﺪ. ﻫﻤﭽﻨﯿﻦ در ﻣﻄﺎﻟﻌﻪ ﺑﺎﻓﺖ ﺷﻨﺎ ﺳﯽ، اﻓﺰاﯾﺶ ﻗﺎﺑﻞﺗﻮﺟﻪ ﻃﻮل ﭘﺮزﻫﺎي روده در ﺗﯿﻤﺎر ﺷﻤﺎره ﺳﻪ ﺑﺎ 40 گرم بر ﮐﯿﻠﻮﮔﺮم ﻣﻮﻧﺖ ﻣﻮرﯾﻠﻮﻧﯿﺖ ﻧﺴﺒﺖ ﺑﻪ ﺳﺎﯾﺮ ﺗﯿﻤﺎرﻫﺎ ﮐﺎﻣﻼً ﻣﺸﻬﻮد ﺑﻮد.

In the present study, the effect of Montmorillonite (MMT) on growth performance and intestinal morphology in Rainbow trout (Oncorhynchus mykiss) in Chaharmahal and Bakhtiari Province has been evaluated. Fish were fed commercial diet for 7 days to adapt to the water environment. Then, normal fish (without malformation and fin rot) with average weight of 20 grams were divided randomly into three groups with 3 replicate. Each group was fed with a specific diet for 8 weeks. Dietary MMT was added in two levels of 20, 40 g /kg for treatment groups while control group was fed without dietary MMT. It is found that all treatment groups had significantly highest increase in length and weight in comparison to control group but there were not any significant differences in feed conversion ratio (FCR) and growth performance. Also length of intestinal microvilli was significantly higher in group 3 with 40 g /kg MMT in comparison to other groups.