پديدآورندگان :
Aldaghi Leila Sadat Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran , Rad Abolfazl Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran , Arab Atefeh Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran , Kasaeeian Jamal Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran , Iranshahi Mehrdad Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran , Shahir Sadr Ahmad Bioinformatics Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran; Bioinformatics Research Center, Cheragh Medical institute Hospital, Kabul, Afghanistan , Soltani Fatemeh Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
چكيده فارسي :
Cancer is one of the leading causes of death worldwide. Despite certain advances in cancer therapy, still there is considerable demand for developing efficient therapeutic agents. Nowadays, there is a rising interest in the use of natural-based anti-cancer drugs. In this study, the cytotoxicity of farnesiferol C and microlobin isolated from Ferula szowitsiana was investigated against MCF-7, HeLa and KYSE cancer cell lines. In addition, the mechanism of binding of these compounds to apoptotic proteins (Bax, Bak and Bcl-2) was analyzed by an in silico method.
We used MTT assay in order to assess the cytotoxicity of compounds against cancer cell lines. For in silico study, the AutoDock 4 was adopted.
According to the in vitro findings, in general, farnesiferol C showed significant cytotoxicity at higher concentrations ( 50 µM) following 48 and 72 h incubation with the selected cancer cells; however, microlobin exhibited almost no activity at concentrations up to 100 µM. The in silico results revealed that both compounds could bind to Bax more efficiently rather than to Bcl-2 or Bak proteins.
The results obtained by our preliminary in vitro and in silico studies suggest that these compounds might induce apoptosis through Bax activation; however further studies, either in vitro or in vivo are needed to clarify these activities.
