High sensitive voltametric nanosensors for determination of Xanthine in the prensece of Caffeine in fish samples

Caffeine is a central nervous system stimulant of the methylxanthine class. It is the world s most widely consumed psychoactive drug. Unlike many other psychoactive substances, it is legal and unregulated in nearly all parts of the world. The most prominent is that it reversibly blocks the action of adenosine on its receptor and consequently prevents the onset of drowsiness induced by adenosine. Caffeine also stimulates certain portions of the autonomic nervous system. In this work, CuO/CNTs nanocomposite with room tempratured ionic liquid carbon paste electrode fabricated for determination of xanthine in the presences of caffein in food samples. Nanopowders was characterized by SEM, and TEM. The results obtained confirm the presence of CuO in nanopowders produced during chemical precipitation. In continuous, Electrochemical behavior of caffein and xanthine at the CuO/CNTs/IL /CPE had been investigated in phosphate buffer solution (PBS) by cyclic voltammetry (CV).other voltametric techniques such as chronoamperometry (CA) and differential pulse voltammetry (DPV) were investigated. The experimental results shows wide linear dynamic range and ofcourse good detection limit was found to be 5.0 nM. The proposed sensor was successfully applied for the determination of xanthine in the prensece of caffein in fish samples.