Antibacterial effect of Aluminum zirconium oxide nanocomposite against clinical pathogens

Nano-materials are the more efficient, since they are able to attach more copies of microbial molecules and cells [1]. Nanoscale materials have been investigated for antimicrobial activity as growth inhibitors [2], killing agents [3] or antibiotic carriers [4]. The objective of this study was to evaluate the antibacterial potential of Aluminum zirconium oxide nanocomposite (Al2Zr3O9) against a clinical isolates of Pseudomonas aeruginos. Pseudomonas aeruginosa has become an important cause of gram-negative infection, especially in patients with compromised host defense mechanisms. It is the most common pathogen isolated from patients who have been hospitalized longer than 1 week, and it is a frequent cause of nosocomial infections. Pseudomonal infections are complicated and can be life-threatening. In this study the antibacterial activities of nanocomposite (Al2Zr3O9) was investigated against a clinical isolates of Pseudomonas aeruginosa using agar well diffusion method. Briefly, 6mm-diameter wells were made on the surface of solid medium using a sterile cork borer. Aluminum zirconium oxide nanocomposite was dissolved in Dimethyl sulfoxide (DMSO) and a solution with 1mg/ml concentration was prepared. Nanocomposite solution and DMSO (Dimethyl sulfoxide was used as negative control) separately placed in 6mm diameter-wells and incubated at 37 ± 2◦C for 24h. Final results were evaluated by measuring the average diameter of inhibition zone around each well. The results show that zoon inhibition of Aluminum zirconium oxide nanocomposite was 12mm (Fig1). These results confirm the potential of Aluminum zirconium oxide nanocomposite as new antimicrobial materials.