پديدآورندگان :
fiezabadi Mina M.feizabadi@std.du.ac.ir School of Chemistry, Damghan University, 3671641167, Damghan, Iran; E-mail: , Ajloo Davood School of Chemistry, Damghan University, 3671641167, Damghan , Soleymanpour Ahmad School of Chemistry, Damghan University, 3671641167, Damghan, Iran , Faridnouri Hassan School of Biology, Damghan University, 3671641167, Damghan, Iran , Zare Nazarzadeh School of Chemistry, Damghan University, 3671641167, Damghan, Iran
چكيده فارسي :
Fast and easy determination and measurement of hydrogen peroxide (H2O2) is of practical importance in clinical, pharmaceutical, biochemical, environmental [1,2]. In recent years, researchers have focused on magnetic nanoparticles. Due to their good biocompatibility, strong superparamagnetic properties, low toxicity and easy preparation processes, magnetic nanoparticles have been used in various fields, including enzyme immobilization [3]. However, the problems of aggregation and rapid biodegradation limited applications of magnetic nanoparticles to biosensing [4]. One of the most notable candidates for improvement of such practical applications may be nanomaterials based on conducting polymers. In the present study, Fe3O4 nanoparticles and conductive carboxy methyl cellulose/Fe3O4/CNT-COOH (CMC-Fe3O4-CNT-COOH), synthesized by organic chemistry group, were combined to develop an immobilization of horseradish peroxidase enzyme (HRP) for the determination of H2O2 by using electrochemical methods. The proposed biosensor showed good reproducibility and high sensitivity to H2O2 with the detection limit of 0.63 µM (S/N=3). In the range of 3.0–80.0 µM, the catalytic reduction current of H2O2 was proportional to its concentration. The apparent Michaelis–Menten constant of HRP on the CMC-Fe3O4-CNTCOOH nanocomposite was estimated to be 0.58 µM.
