بررسي نقش جنسيت و هشت هفته تمرين استقامتي بر سطح آيريزين سرمي در رتهاي نر و ماده

Evaluation of gender role and eight-week endurance training on serum irisin level in male and female rats

هدف از انجام پژوهش حاضر ، بررسي نقش جنسيت و هشت هفته تمرين استقامتي بر سطح آيريزين سرمي در رتهاي نر و ماده نژاد ويستار بود . بدين منظور 24 سر موش ( 12سر نر و 12 سر ماده) نژاد ويستار با دامنه وزني 30 ±250 گرم و سن هشت هفته به طور تصادفي به 4گروه شش تايي تقسيم شدند . گروهها شامل : گروه كنترل نر ، گروه كنترل ماده ، گروه تمريني نر و گروه تمريني ماده بودند . گروههاي تمريني به مدت 8 هفته و هفته اي سه جلسه ، تمرينات استقامتي را بروي تردميل مخصوص حيوانات انجام دادند. تمرينات از 15 دقيقه در هر جلسه و با سرعت 16 متر در دقيقه در هفته اول آغاز و سپس به 45 دقيقه در هر جلسه با سرعت 26 متر در دقيقه در هفته هشتم رسيد. نمونه هاي خوني از طريق خونگيري از ناحيه قلب انجام گرفت و در لوله هاي آزمايشگاهي هپارين دار براي سانتريفيوژن جمع آوري شد . جهت اندازه گيري ميزان آيريزين سرمي از روش ELISA استفاده شد . از آزمون كولموگروف اسميرنوف براي بررسي نرمال بودن داده‌ها ، آزمون لون جهت بررسي همگني واريانس ها و آزمونT مستقل جهت بررسي اثر گروه و تحليل واريانس يكراهه جهت مقايسه بين گروهي و از نرم افزار SPSS نسخه 21 جهت بررسي نتايج استفاده گرديد. سطح معناداري P

The aim of this study was to investigate the role of gender and eight-week endurance training on serum irisin levels in male and female Wistar rats. For this purpose, 24 mice (12 male and 12 female) Wistar rats weighing 30 ± 250 gr and eight weeks old were randomly divided into 4 groups of six. The groups included male control group, female control group, male training group and female training group. The rats in exercise group were trained to run on the treadmill 3 days in a week for 8 weeks. The exercises started from 15 minutes per session and started at 16 meters per minute in the first week and then reached 45 minutes per session at 26 meters per minute in the eighth week. Blood samples were taken from the heart area by blood sampling and collected in heparin tubes for centrifuge digestion .ELISA was used to measure serum irisin. The Kolmogorov-Smirnov test was used to check the normality of the data. The Lone test was used to examine the homogeneity of variances and independent T-test to examine the effect of the group and one way ANOVA to compare the intergroup and SPSS software version 21 to test the results. The significance level was considered as P 0.5 .The results showed that there was a significant difference between male and female rats in irisin secretion , which was significantly higher in male rats than female rats. Endurance training significantly increased serum irisin levels in male and female rats as compared to control group. This increase in female rats was higher than male rats