Preparation of Functionalized Graphene Oxide Loaded with Copper Oxide Nanoparticles and Investigation of Its Antibacterial Activities

Metal oxide nanoparticles are a particular class of nanomaterials that have garnered huge interest in the field of scientific research, and in many other technical disciplines, due to their chemical and physical properties and the numerous effective applications [1]. Copper oxide nanoparticles (CuO NPs) with potential effects and large surface area have superior chemical and physical properties that are noticeably different from their bulk materials. CuO NPs show a range of useful properties, such as high temperature superconductivity, relative stability, low cost, antibacterial and antiviral activities [2]. CuO NPs has antibacterial activity against gram negative and gram positive bacteria. Some of the most problematic multi drug resistant (MDR) microorganisms that are encountered currently include Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia [3]. In this article, the activity of the Gram-negative (E.coli) and Gram-positive bacteria (Staphylococcus aureus) was investigated by determination of zone diameter. Firstly, the GO-PPD was prepared by reaction of GO with p-phenylenediamine under nitrogen atomospher, and then treated with cyanuric chloride and ethylenediamine and cupric chloride, to obtain GO(PPD-CC-EN) nanocomposite. Antibacterial activities of (GO-PPD-CC-EN)-CuO nanocomposite was determined. It has been shown that these nanocomposite have good effect on both gram negative and gram positive bacteria in comparison to the other antibacterial substances.