اثر ژن OsNAC بر روي بيان ساير ژنهاي برنج تحت تنش شوري با استفاده از راهكار OsNAC

Effect of OsNAC6 on rice gene expression under salinity stress by a bioinformatic approach

فاكتورهاي رونويسي، تنظيم كننده هاي اصلي تغيير در بيان ژن و پاسخ به تنش هاي محيطي هستند.ژن هاي NAC يكي از بزرگترين خانواده هاي فاكتورهاي رونويسي است كه تا به امروز تنها در گياهان يافت شده و تحت تنش شوري و خشكي بيان شده و باعث سازگاري به تنش مي شوند.با بررسي ژن هاي هم بيان با ژن OsNAC در گياه برنج تحت شرايط شورريمشخص گرديد كه ژن OsNAC بر روي ژن هاي hairpin و كينارها تاثير مي گذارد و خود اين ژن باعث القاي بيان عوامل رونويسي از جمله wrky و كيناز ها مي شود

The OsNAC6 gene is a member of the NAC transcription factor gene family in rice. Expression of OsNAC6 is induced by abiotic stresses, including cold, drought and high salinity. OsNAC6 gene expression is also induce by wounding and blast disease .The results of microarray analysis revealed that many genes that are inducible by abiotic and biotic stresses were up regulated in rice plants overexpressing OsNAC6. A transient transactivation assay showed that OsNAC6 activates the expression of at least two genes, including a gene encoding peroxidase. Collectively, these results indicate that OsNAC6 functions as a transcriptional activator in response to abiotic and biotic stresses in plants. We conclude that OsNAC6 may serve as a useful biotechnological tool for the improvement of stress tolerance in various kinds of plants