شماره ركورد كنفرانس :
4818
عنوان مقاله :
Comparing of ELISA and RT-PCR methods for detection of quince viruses
عنوان به زبان ديگر :
Comparing of ELISA and RT-PCR methods for detection of quince viruses
پديدآورندگان :
Farhadi Tooli Sakineh sakineh.farhadi@gmail.com Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran, P.O. Box: 31535-1897 , Jafarkhani Kermani Maryam Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran, P.O. Box: 31535-1897 , Ghanbari Alireza Faculty of Agriculture and Natural Resources,University of Mohaghegh Ardabili, Ardabil , Zeinalabedini Mehrshad Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran, P.O. Box: 31535-1897 , Mohammad Naji Amir Faculty of Agriculture Shahed University, Tehran
كليدواژه :
DAS , ELISA , RT , PCR , Quince , Virus detection
عنوان كنفرانس :
سومين همايش بين المللي و يازدهمين همايش ملي بيوتكنولوژي جمهوري اسلامي ايران
چكيده فارسي :
The most important viral diseases that commercially affect pome fruits trees are: Apple chlorotic leaf spot virus (ACLSV), Apple mosaic virus (ApMV), Apple stem grooving virus(ASGV), Apple stem pitting virus (ASPV), Tomato ringspot virus (TORSV) and Tobacco ring spot virus (TRSV). The first four viruses have the greatest impact on the quality and quantity of quince’s production in orchards of Iran. Early and accurate detection of viral pathogens is undoubtedly essential to control the spread of the virus and ensure the production of healthy plants for cultivation. In this study, viral detection methods (DAS ELISA and RT PCR) were compared in cultivars (IsfahanSour, Isfahan and Neyshabur) and two commercial rootstocks (QA and QC) collected from Kamal-Abad collection in Karaj, Iran. The DAS-ELISA was not capable of detecting viruses in the cultivars and rootstocks studied. However, the RT-PCR molecular method illustrated contamination of commercial rootstocks (QA and QC) and cultivars (Sour Isfahan and Neyshabur) to ACLS virus. ASG was observed in commercial rootstocks (QA and QC), but none of the plants showed contaminated by ASPV, ApMV, TORSV and TRSV viruses.
