(Optimization of antibacterial pigment production in an indigenous isolated bacteria from Iran by response surface methodology (RSM)

(Optimization of antibacterial pigment production in an indigenous isolated bacteria from Iran by response surface methodology (RSM

In recent years, there is a great interest for production and development of natural pigments. Many microorganisms, including bacteria and fungi, can be used for the industrial production of various pigments by using fermentation technology. Microbial pigments have broad area of applications, mainly in food, pharmaceuticals and textile industries. In this study, optimum culture conditions for growth and antibacterial pigment production in a newly isolated soil bacteria from Iran, N-1-1, were evaluated using the response surface methodology (RSM) in the Box-Behnken design (BBD). The effects of three factors including pH (4, 6, 8), NAOH (%4, %6, %8) and temperature (25, 28, 31°C) was studied in a fixed concentration of inoculum under agitation. The maximum pigment production, 0.858 gr/L, was observed at 25°C, pH=6 and NAOH %4 after 48 hours in a shake flask 50 ml. Pigment of N-1-1 strain showed antibacterial properties to Staphylococcus aureus, Pseudomonas aeruginosa, basillus subtilis and the minimum inhibitory concentration (MIC) was 152, 134 and 64 mg/ml, respectively. Further studies are undergoing to determine other culture conditions for increasing the pigment production, as well as to identify and characterize the physico-chemical properties of its antibacterial compounds.