(Apolipoprotein E (ApoE) genotyping in diabetic patients with cardiovascular diseases (CVD

Apolipoprotein E (ApoE) genotyping in diabetic patients with cardiovascular diseases (CVD)

Apolipoprotein E (ApoE) is one of the most reviewed lipoproteins which has rules in lipid metabolism. Its gene is polymorphic with three alleles, and the two polymorphic sites are codon 112 and 158 which codes Cysteine and Arginine respectively. There are so many methods for genotyping of this locus, which in this study we use ARMS PCR with 5 primers. The three most common allelic forms of this gene are ε2, ε3, and ε4. According to previous studies, ε2 is a protective allele against CVD and ε4 is not. Also, ε4 has its rules in Insulin resistance in Diabetes especially type 2, So ApoE genotyping has been had vital importance. For genotyping ApoE gene, 107 blood samples of diabetic patients with CVD in Kashan city were collected, and amplified with an ARMS PCR to investigate six genotypes separately. The frequencies of alleles were 3,81 and 15 percent for ε2, ε3, and ε4 respectively and E3E3 genotype was the most in the population. Regarding the similarities of frequencies between different populations we used statistical analysis. The frequencies of alleles in Kashan diabetic with CVD population differs from the other studies in Iran but its similar to populations like China, Norway and Saudi Arabia. In conclusion this study shows that the ARMS PCR with 5 primers is an efficient method for ApoE genotyping and the frequencies of alleles in this population is similar to most of the normal population in the world, so it can be helpful To predict and prevent diseases associated with this genotype