Effect of Chlorella vulgaris on Methylene blue uptake

INTRODUCTION: Nowadays, the u se of synthetic dyes in different industries causes the pollution of aquatic habitats. Additionally, these synthetic dyes are hazardous to human and animals and can cause a reduction i n photosynthetic activities due to the decrease in light penetration. The use of microorgani sms in order to biosorption of dye is an effec tive and economical way in comparison wi th other conventional methods. Among a number of microorganisms, microalgae are the favourable agents that their influence in the removal of toxic dyes has been proved.[1, 2] AIMS AND OBJECTIVES: This study focused on the evaluation of the effect of Chlorella vulgaris (the green unicellular photosy nthetic microalga) to remove Methylene Blue (a synthetic dye) from aqueous solution. MATERIALS AND METHODS: A Methylene Blue (MB) stock solution was provided and a few solutions with the initial concentrations of 25, 50, 75, and 100 mg l-1were prepared by diluting the stock solution with distillled water and the pH value was adjusted to 7 for each solution. 100 and 1000 mg of microal gae were added to each solution and stirred at 180 rpm for 1 hour. Then, samples were centrifuged at 5000 rpm and 4 ºc for 10 min. Finally, the su pernatant was discarded to analyse spectrophoto meterically at the 665nm. The amount of dye uptake (mg g-1) was calculated according to: qe= (c i-cf) v/w qe (mg MB.gr-1 microalga): MB uptak e, Ci (mg l-1): initial MB concentration, Cf (mg l-1): final MB concentration, W (g): dry weight o f the Chlorella vulgaris VL: volume of the solutio n[3, 4] RESULTS DISCOSSION: In the present work, the amount of dye adsorption a t various microalgae and dye concentrations were investigated. The results revealed that Chlorella vulgaris had the desirable impact on MB uptake from the solution. Based on Fig. 1 the lower concentration of microalgae (100ppm) had more effective impact on the dye adsorption process. This showed that there was not the linear relationship between the increase in dye concentration and raise the microalgae concentration during the dye bioadsorption process.

INTRODUCTION: Nowadays, the u se of synthetic dyes in different industries causes the pollution of aquatic habitats. Additionally, these synthetic dyes are hazardous to human and animals and can cause a reduction i n photosynthetic activities due to the decrease in light penetration. The use of microorgani sms in order to biosorption of dye is an effec tive and economical way in comparison wi th other conventional methods. Among a number of microorganisms, microalgae are the favourable agents that their influence in the removal of toxic dyes has been proved.[1, 2] AIMS AND OBJECTIVES: This study focused on the evaluation of the effect of Chlorella vulgaris (the green unicellular photosy nthetic microalga) to remove Methylene Blue (a synthetic dye) from aqueous solution. MATERIALS AND METHODS: A Methylene Blue (MB) stock solution was provided and a few solutions with the initial concentrations of 25, 50, 75, and 100 mg l-1were prepared by diluting the stock solution with distillled water and the pH value was adjusted to 7 for each solution. 100 and 1000 mg of microal gae were added to each solution and stirred at 180 rpm for 1 hour. Then, samples were centrifuged at 5000 rpm and 4 ºc for 10 min. Finally, the su pernatant was discarded to analyse spectrophoto meterically at the 665nm. The amount of dye uptake (mg g-1) was calculated according to: qe= (c i-cf) v/w qe (mg MB.gr-1 microalga): MB uptak e, Ci (mg l-1): initial MB concentration, Cf (mg l-1): final MB concentration, W (g): dry weight o f the Chlorella vulgaris VL: volume of the solutio n[3, 4] RESULTS DISCOSSION: In the present work, the amount of dye adsorption a t various microalgae and dye concentrations were investigated. The results revealed that Chlorella vulgaris had the desirable impact on MB uptake from the solution. Based on Fig. 1 the lower concentration of microalgae (100ppm) had more effective impact on the dye adsorption process. This showed that there was not the linear relationship between the increase in dye concentration and raise the microalgae concentration during the dye bioadsorption process.