پديدآورندگان :
Bohlooli Arkhazloo Rozita Department of Chemistry, University of Mohaghegh Ardabili, Ardabil , Eskandari Habibollah heskandari@uma.ac.ir Department of Chemistry, University of Mohaghegh Ardabili, Ardabil;
كليدواژه :
Magnetic poly (1 , vinylimidazole) , Ni(II) , Sulfasalazine , trace determination
چكيده فارسي :
Pharmaceuticals are produced in huge levels. They may be leaked to the environment from the factories or/and homes, and may produce many problems for humans, living creatures and ecosystems. Therefore, development of efficient monitoring methods to understand the levels of the pollutions is mandatory to control the sources of the leakages.Sulfasalazine (SZ) is an effective pharmaceutical for rheumatoid arthritis. Also, it controls active ulcerative colitis or crohn’s disease. However, it can produce adverse reactions which may lead to disorder of the hemopoietic system and potential carcinogenic effects. SZ has been studied and determined in various samples by high performance liquid chromatography, spectrophotometry and luminescence spectroscopy [1].In this study, a new magnetic solid phase extraction method has been developed for SZ determination. The surfaces of magnetite nanoparticles (MNPs) were modified by 3-(trimethoxysilyl)propyl methacrylate as a linker. Then, the modified MNPs were used as the magnetic cores to prepare a magnetic (1-vinylimidazole/divinylbenzene) polymer. The magnetic polymer was characterized by scanning electron microscopy, transmission electron microscopy, FT-IR, vibrating sample magnetometery and X-ray diffraction spectroscopy. Due to the electron donating of the nitrogen atoms in imidazole moieties, Ni(II) can be adsorbed by the magnetic nano-polymer. Sulafasalazine contains salycilate moieties capable to be adsorbed by the magnetic polymer through mixed ligand complexation via adsorbed Ni(II) ions. Effects of the affective parameters on the solid phase extraction process such as amount of adsorbent, pH, extraction time and the others were investigated. The eluted sulfasalazine (by an ethanolic solution) was monitored by spectrophotometry at 450 nm. Under optimal conditions, the calibration curve was obtained in the range of 0.04-1.4 µmol L-1. The limit of detection for the sulfasalazine determination method was found as 0.008 μmol L-1. Relative standard deviation and recovery for analysis of an aqueous solution containing 0.1 μmol L-1 of sulfasalazine were of 4.1 and 97.0%, respectively. Different river and lake water samples were successfully analyzed by the method.
